食品与机械2012,Vol.28Issue(3):144-147,150,5.DOI:10.3969/j.issn.1003-5788.2012.03.039
泰和乌鸡黑色素提取工艺的研究
Comparative study on extraction process of Taihe silky fowl melanin
朱方 1倪治明 1张莹 1朱加进1
作者信息
- 1. 浙江大学生物系统工程与食品科学学院,浙江杭州310029
- 折叠
摘要
Abstract
Comparative study on extraction process of Taihe silky fowl melanin(SF-M) with enzymatic and hydrochloric acid methods. The optimum extraction conditions of SF-M were determined by single-factor experiments and orthogonal tests of enzymatic and hydrochloric acid methods,besides,anti-free radical properties, light and heat stabilities of SF-M from two extraction methods were studied and compared. The results showed that the optimum extraction condition of SF-M with enzymatic method was obtained as follows; the use of papain, pH 6.0,temperature 65℃, the ratio of material to liquid 1:2(m:V),the ratio of enzyme to substrate 1:25(m:m) for 4 hours; the optimum extraction condition of SF-M with hydrochloric acid method was the ratio of meal to liquid 1:4(m:V), reflux temperature 95℃ . extraction time 2 hours; the extraction ratio of SF-M with enzymatic extraction was higher than the hydrochloric acid extraction, the anti-free radical properties and the heat stability of SF-M with enzymatic extraction were better than the hydrochloric acid extraction. It illustrated that the enzymatic extraction method was better than hydrochloric acid extraction melhod.关键词
乌鸡/黑色素/酶解提取/盐酸提取Key words
silky fowl/ silky fowl melanm(SF-M)/enzymatic extraction/ hydrochloric acid extraction引用本文复制引用
朱方,倪治明,张莹,朱加进..泰和乌鸡黑色素提取工艺的研究[J].食品与机械,2012,28(3):144-147,150,5.