中国食品添加剂Issue(3)：88-92,5.
一株产单宁酶酵母菌株的筛选
Screening of Tannase-producing strains
蔡淑娟 1赵祥颖 2刘建军 1杨丽萍 2乔君2
作者信息
- 1. 山东轻工业学院食品与生物工程学院,济南250353
- 2. 山东省食品发酵工程重点实验室,济南250013
- 折叠
摘要
Abstract
Tanin acyl hydrolase (E. C. 3.11.20) commonly referred to as tannase, catalyzes the hydrolysis of ester and despide bonds in hydrolysable tannins, releasing glucose and gallic acid. In this study, 69 tannase - producing yeast strains were isolated by using enrichment - culture technique from natural resources, such as persimmons, unrip-en bananas and the tea leave or soil of the tea trees. The characteristic and tannase - producing activity of the samples were investigated. Finally, one yeast strain FG6 -1 with higher tannase activity was obtained. Before the optimization of the conditions of fermentation, the tannase - producing activity of this yeast strain FC6 - 1 was 2. 86U/mL This strain was able to hydrolyze tannin into gallic acid which was determined by thin layer chromatography (TLC) and glucose which was determined by Biosensor Analyzer.关键词
单宁酶/筛选/单宁酸/没食子酸/葡萄糖Key words
Tannase/ screening/ tannin/ gallic acid/ glucose
分类
轻工纺织引用本文复制引用
蔡淑娟,赵祥颖,刘建军,杨丽萍,乔君..一株产单宁酶酵母菌株的筛选[J].中国食品添加剂,2012,(3):88-92,5.
