中国食品添加剂Issue(3)：137-142,6.
高产2-苯乙醇酵母菌株的筛选及培养基优化
Screening of high - yield yeast strains for 2 - phenylethanol land its medium optimization
摘要
Abstract
2 - phenylethanol is an aromatic alcohol with a rose - like odor. It is a very important fragrance used in the cosmetic and tobacco industries and is also largely used in food industries. More and more people like 2 - phenythanol from microorganism than synthetic from chemicals. In one - phase fermentation, the yield of 2 - phenylethanol by single strain of yeasts are 3. 6g/L and 3. 8g/L in China and abroad respectively. Two strains were selected during the initial screen: a strain of Saccharomyces cerevisiae TYQ which could produce 1.6886g/L 2 - phenylethanol and another strain of Geotrichum candidum GS10A which could produce 1.0025g/L 2 - phenylethanol. Medium of the TYQ was optimized by the single factor test and orthogonal test. The optimized medium contains glucose 80g/L, L - phenylalanine 35g/L, yeast extract powder l0g/L, peptone l0g/L Grown under the condition of 10% (v/v)inoculation, 28℃ and 120i/min for 36h, the yeast TYQ yielded 2 - phenylethanol of 4.4025g/L关键词
2-苯乙醇/酿酒酵母/白地霉/培养基优化Key words
2-phenylethanol/ saccharomyces cerevisiae/ geotrichum candidum/ medium optimization分类
轻工纺织引用本文复制引用
唐育岐,刘天明,赵修报,刘波,王红,刘思喜..高产2-苯乙醇酵母菌株的筛选及培养基优化[J].中国食品添加剂,2012,(3):137-142,6.基金项目
国家自然科学基金资助(30970091,30900039). （30970091,30900039）
