中国食品学报2012，Vol.12Issue(5)：82-87,6.
保加利亚乳杆菌菊芋复合汁增菌培养基的优化筛选
Screening and Optimization of Compound Helianthus Tuberosus Juice Medium of Lactobacillus Bulgaricus
摘要
Abstract
Helianthus tuberosus was chosen as the main raw material and LactobaciUus bulgaricus Lb-DR which was isolated from dairy products was used as experimental strains in this paper. The influence of adding different nutriment in helianthus tuberosua juice medium on the growth of Lactobacillui bulgaricus Lb-DR was studied. The optimum compound enrichment medium of Lb-DR were selected through orthogonality test. The results showed that the growth of Lb-DR could be promoted obviously(P<0.01) by adding tomato Juice, lactose, byptone, CaCO3 into helianthus tuberosus juice compound enrichment medium. The optimal proportion of the enrichment medium wag obtained by using orthogonality test, which consisted of 7.5% tomato Juice, 1.5% lactose, 1% tryptone, 0.3% CaCO3 in helianthus tuberosus juice. The viable counts of Lb-DR reached 1.50xl0'cfu/mL after incubated in the optimum enrichment medium for 16 hours at 37 ℃, which was 23.96 times of that with the contrast medium. Compared with MRS medium, the viable counts of Lb-DR in the two kinds of mediums were at the same level, however, the cost of compound helianthus tuberosus juice medium could be decreased by 2400 yuan/t.关键词
保加利亚乳杆菌/菊芋汁/增菌培养基/优化筛选Key words
LactobaciUus bulgaricus/ helianthus tuberosua juice/ enrichment medium/ screening and optimizing引用本文复制引用
李冲,谷新晰,田晶晶,田洪涛,许文涛,罗云波..保加利亚乳杆菌菊芋复合汁增菌培养基的优化筛选[J].中国食品学报,2012,12(5):82-87,6.基金项目
河北省农副产品加工重大科技专项(03220171D) （03220171D）
