包装与食品机械2012，Vol.30Issue(3)：18-22,5.DOI:10.3969/j.issn.1005-1295.2012.03.005
黑曲霉耐酸性α-淀粉酶的固态发酵条件研究
Solid State Fermentation of Acid Resistant α-Amylase from Aspergillus Niger
摘要
Abstract
Solid state fermentation conditions of acid amylase from Aspergillus niger PZ30I were studied. The results showed that the optimal medium contained wheat bran 7. Og, glucose 0. 07g, starch 0. 21g, (NH4)2SO40.07g,optimal initial pH 4.2. Based on the above medium, PZ301 was cultured at 35? for 72 hours with 1:1 of wheat bran to water and inoculation 3mL( 107spores/mL) ,enzyme activity was 19. 6 lU/g dry medium. The biomass curve of PZ301 in solid slate fermentation was determined, and production kinetics was studied through studying the relation between the curve of strain PZ301 and the time of acid stable a-amyl-ase production. The result indicated that the synthesizing type of acid stable ot-amylase was synchronous synthesis model.关键词
黑曲霉PZ301/酸性α-淀粉酶/固态发酵/同步合成型Key words
Aspergillus niger PZ301 /acid resistant ot-amylase/solid state fermentation/synchronous synthesis model分类
轻工纺织引用本文复制引用
吴茜茜,王红梅,张增辉,蔡敬民..黑曲霉耐酸性α-淀粉酶的固态发酵条件研究[J].包装与食品机械,2012,30(3):18-22,5.基金项目
安徽省教育厅重点项目(KJ2012A246) （KJ2012A246）
教育部第三批高等学校特色专业建设点项目 （）
