摘要
Abstract
A new method for determining benzo （a） pyrene in vegetable oil by high -performance liquid chromatography with liquid -liquid extraction as pre -process was researched. The sample was extracted with acetonitrile, separated by reversed phase column C18 column（5 μm,4.6 mm × 250 mm i d） , the mobile phase acetonitrile- water（88： 12） with velocity of flow 1.0 mL/min, excitation wavelength 384 nm, emission wavelength 406 nm, column temperature 30 ℃ and the injection volume 10 μL. The detection limit was 0.5 μg/kg, recoveries 95.1% - 100.6 % , and the relative standard deviation （RSD） 1.78% -2.15%. This method has advantages of simple pre -process, stable operation, low consumption of reagents, short analysis time （ one sample treatment within twenty five minutes） and is suitable for the quantitative and qualitative determination of benzo （a） pyrene in vegetable oil.关键词
苯并（a）芘/液液萃取/高效液相色谱法/植物油Key words
benzo (a) pyrene/liquid - liquid extraction/high performance liquid chromatography/vegetable oil分类
轻工纺织
