食品工业科技2012，Vol.33Issue(14)：87-89,3.
UPLC-MS／MS检测柑桔不同组织中的柠檬苦素
Detection of limonin in citrus with different tissue by UPLC-MS/MS
摘要
Abstract
Objective：Determined the contents of Limonin in citrus peel,citrus flesh,dried tangerine fibre and citrus seed. Analysis of the source of citrus bitterness substances in processing. Methods-The column was waters ACQUITY BEH C18（50mm ×2.5mm, 1.9μm） by UPLC-MS/MS, mobile phase ,, using gradient elution, A was acetonitrile and B was water,A： 10%（0min）-50%（3min）-10%（4min）-10%（5min）,the velocity of flow was 0.3mL/min,column temperature was 40℃, injection quantity was 5tJL, detection time was 5min, ESI （＋） scan,the limonin＇s parents were m/z=471.1 ,daughter were m/z=425.3 and m/z=161.1. Results .. Limonin could be detected in the chromatography-mass spectrum conditions. The limonin contents discretion order in citrus was citrus seed 〉dried tangerine fibre〉citrus peel〉citrus flesh. Conclusion ： In citrus processing, tried to choose seedless varieties. Using manual skinnino.and eliminate dried tanaerine fibre could avnicl the. bitter关键词
UPLC-MS／MS/测定分析/柑桔/组织/柠檬苦素Key words
U PLC- MS/MS/determination/ctrus/tissue/limonin分类
轻工纺织引用本文复制引用
江海,李新生,吴三桥,刘新,韩豪,高玥,彭浩..UPLC-MS／MS检测柑桔不同组织中的柠檬苦素[J].食品工业科技,2012,33(14):87-89,3.基金项目
陕西省教育厅科研项目 （）
陕西理工学院科研资助项目（SLG0814）. （）
