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多菌复合酸菜发酵剂工业化培养基的优化

韩德权 许永君 吴桐 杨丽娟 雷虹 孙庆申

食品工业科技2012,Vol.33Issue(14):179-182,4.
食品工业科技2012,Vol.33Issue(14):179-182,4.

多菌复合酸菜发酵剂工业化培养基的优化

Optimization of industrial medium for multi-bacteria complex sauerkraut fermentation agent

韩德权 1许永君 2吴桐 1杨丽娟 2雷虹 1孙庆申1

作者信息

  • 1. 黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,黑龙江哈尔滨150080 农业微生物技术教育部工程研究中心,黑龙江呼兰150500
  • 2. 黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,黑龙江哈尔滨150080
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摘要

Abstract

In order to meet the requirements of industrial production,this article optimized the fermentation medium of multi-bacteria complex sauerkraut fermentation agent using cheap raw materials. Mono-factor tests and orthogonal experimental design were used to select the optimal amount of different components in the medium based on the mixed fermentation. Considering the biomass yield and product yield factors,the optimal combination of medium was determined as follows..2.5% corn saccharification solution,1.5% soybean meal hydrolyzate,0.5% corn steep liquor,2g/L K2HPO4,0.5g/L MgSO4,20mg/L MnSO4,under which the number of viable cells was 2,06× 10^9CFU/mL. The ratio of strains was Lactobacillus paracasei(L1 ):Lactobacillus buchneri (L5):Bacillus coagulans(B.c)=1:2.12:2.87,which was very close to the expected proportions of the three strains as determined in our lab previously.

关键词

复合酸菜发酵剂/工业化培养基/正交实验

Key words

complex sauerkraut fermentation agent/industrial medium/orthogonal test

分类

轻工纺织

引用本文复制引用

韩德权,许永君,吴桐,杨丽娟,雷虹,孙庆申..多菌复合酸菜发酵剂工业化培养基的优化[J].食品工业科技,2012,33(14):179-182,4.

基金项目

黑龙江省教育厅面上项目(11551360). ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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