食品工业科技2012，Vol.33Issue(14)：192-195,4.
响应面优化酶催化米糠油甘油解制备甘油二酯
Optimization of diacylglycerol production from lipase-catalyzed glycerolysis of rice bran oil by response surface methodology
摘要
Abstract
Response surface methodology （RSM） was applied to optimize the diacylglycerol （DAG） production from Novozym 435-catalyzed glycerolysis of rice bran oil in t-Butanol system. Based on single-factor analysis, 5-1evel-4-factor central composite rotatable design（CCRD） was employed to evaluate and optimize the effect of DAG synthesis parameters,such as reaction temperature,time,enzyme amount（mass ratio of lipase to rice bran oil）,and substrate mass ratio （rice bran oil：glycerol）. The results indicated that substrate mass ratio was the most important variable. Considering the economical efficiency as well as optimization,the optimum DAG synthesis conditions with the yield of 53.08% were as follows.reaction temperature 60℃,time 10.5h,enzyme amount 10.66%,substrate mass ratio 16.关键词
响应面/甘油二酯/米糠油/甘油解Key words
RSM/DAG/rice bran oil/glycerolysis分类
轻工纺织引用本文复制引用
王诗路,刘辉,咸漠..响应面优化酶催化米糠油甘油解制备甘油二酯[J].食品工业科技,2012,33(14):192-195,4.基金项目
国家自然科学基金青年科学基金项目（21106170）. （）
