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柱色谱分离制备低乙酸乙酯儿茶素

刘伟 郑琳 龚正礼

食品工业科技2012,Vol.33Issue(14):306-309,4.
食品工业科技2012,Vol.33Issue(14):306-309,4.

柱色谱分离制备低乙酸乙酯儿茶素

Column chromatography separation preparation of catechins with low content of ethyl acetate

刘伟 1郑琳 1龚正礼1

作者信息

  • 1. 西南大学食品科学学院,重庆400716
  • 折叠

摘要

Abstract

Catechins is a representative active substance of tea,whose preparation has been much-talked- about. Based on previous study,the thesis hoped to find the optimal control conditions to produce catechins with low content of ethyl acetate through analyzing the separation experiment condition of column chromatography. The best control condition was purifying catechins with polyamide resin and the elution sequence was 25% ethanol, ethyl acetate:80% ethanol=2:1. Product quality index for: ethyl acetate〈20mg/kg, total catechins≥ 75%, EGCG ≥ 55%, Caf〈2%, yield coefficient〉 10%.

关键词

儿茶素/制备/乙酸乙酯/EGCG/咖啡因

Key words

tea catechins/preparation/ethyl acetate/EGCG/caffeine

分类

轻工纺织

引用本文复制引用

刘伟,郑琳,龚正礼..柱色谱分离制备低乙酸乙酯儿茶素[J].食品工业科技,2012,33(14):306-309,4.

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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