食品科学2012，Vol.33Issue(9)：114-117,4.
脉冲电场和pH值协同作用对卵白蛋白微观结构的影响
Effect of PEF Treatment on Secondary Structure of Ovalbumin under Various pH Conditions
摘要
Abstract
The effect of high intensity pulsed electric field（HIPEF） treatment on the secondary structure of ovalbumin under various pH conditions was explored using circular dichroism（CD） spectroscopy.The results indicated that 4 circles of PEF treatment（192μs） at the field intensity of 26.7 kV/cm could induced the unfolding of α-helix structure by 25.43%,23.99% and 42.20%,as well as unfolding of β-sheet structure by 26.20%,15.99% and 38.53% under the conditions of pH 3,7 and 12,respectively.关键词
高强脉冲电场/pH值/卵白蛋白/二级结构/圆二色光谱Key words
high intensity pulsed electric field(HIPEF)/pH/ovalbumin/secondary structure/circular dichroism spectroscopy分类
轻工纺织引用本文复制引用
阳梅芳,曾新安,刘燕燕..脉冲电场和pH值协同作用对卵白蛋白微观结构的影响[J].食品科学,2012,33(9):114-117,4.基金项目
广东省自然科学基金项目 （）
广州市科技攻关计划项目 （）
