食品科学2012，Vol.33Issue(9)：122-126,5.
南瓜皮多糖PP1的提纯、组分分析及原子力显微镜技术观测研究
Extraction,Purification and Properties of Pumpkin Peel Polysaccharide PP1
白娣斯 1马冬 2芮茜 3张静4
作者信息
- 1. 陕西师范大学食品工程与营养科学学院,陕西西安710062 河北联合大学迁安学院,河北迁安064400
- 2. 河北联合大学公共卫生学院,河北唐山063000
- 3. 河北联合大学研究生学院,河北唐山063000
- 4. 陕西师范大学食品工程与营养科学学院,陕西西安710062
- 折叠
摘要
Abstract
A polysaccharide from pumpkin peel,named as PP1,was extracted by hot water extraction method and purified by cetyltrim-ethlammonium bromide（CTAB） method.UV spectroscopy and HPLC were both used to analyze its purity,and its structure and monosaccharide composition were analyzed by IR spectroscopy and GC,respectively.The microstructure of PP1 was observed under an AFM based on fixation with ethanol or Ni2＋.The results showed that PP1 was highly pure and contained neither nuclear acid nor protein.IR spectroscopic analysis indicated that PP1 had characteristic absorption peaks of polysaccharides and pyran ring.Its monosaccharide composition was composed of fructose,arabinose,glucose and galactose at a molar ratio of 0.92：2.76：5.52：2.24.AFM observations showed that its structure was a single chain with no branches.PP1 had a long thin structure when fixed with ethanol but a short fat structure when fixed with Ni2＋.关键词
南瓜皮多糖PP1/原子力显微镜/结构分析Key words
pumpkin peel polysaccharide PP1/atomic force microscope/structural analysis分类
化学化工引用本文复制引用
白娣斯,马冬,芮茜,张静..南瓜皮多糖PP1的提纯、组分分析及原子力显微镜技术观测研究[J].食品科学,2012,33(9):122-126,5.
