食品科学2012，Vol.33Issue(9)：144-148,5.
黑曲霉原生质体诱变选育内切型菊粉酶生产菌株
Screening and Protoplast Mutagenesis of Aspergillus niger for Enhanced Endoinulinase Activity
摘要
Abstract
Soil was collected from the periphery of rotten burdock roots in the burdock planting base of Qinzhuang village,Hekou town,Pei county,Xuzhou municipality.Three batches of Aspergillus niger strains（including Aspergillus niger 3.316 strains） were isolated and 17 strains with an endoinulinase（I）-to-exoinulinase（S） activity ratio of higher than 10 were screened from them by secondary screening.Strain C122803 exhibited the highest I/S ratio（2.78 U/mL） among 17 screened strains.Strain YY18 with an endoinulinase activity of 3.97 U/mL was obtained from strain C122803 through protoplast preparation and LiCl mutagenesis.The activity obtained was 42.80% higher than that of the parent strain.关键词
黑曲霉/菊粉酶/原生质体/诱变Key words
Aspergillus niger/inulase/protoplast/mutagenesis分类
轻工纺织引用本文复制引用
李文利,曹泽虹,董玉玮,孙会刚,刘夕娟,唐盼..黑曲霉原生质体诱变选育内切型菊粉酶生产菌株[J].食品科学,2012,33(9):144-148,5.基金项目
江苏省高校自然科学研究计划项目 （）
江苏省高等学校大学生实践创新训练计划项目 （）
徐州工程学院校级培育项目 （）
