食品科学2012，Vol.33Issue(9)：163-166,4.
复合酶法水解豆粕制备ACE抑制肽
Preparation of Angiotensin-Ⅰ-Converting Enzyme Inhibitory Peptides by Multienzymatic Hydrolysis of Soybean Meal
摘要
Abstract
In order to improve the ACE inhibitory rate of hydrolyzed soybean meal,alkaline protease was used in combination with neutral protease,compound protease,trypsin or flavorzyme for stepwise hydrolysis of soybean meal.The results showed that the highest hydrolysis degree,46.83%,was achieved when soybean meal was sequentially hydrolyzed with alkaline protease followed by trypsin.Further investigations were carried out to optimize the hydrolysis process using response surface analysis.The optimal hydrolysis process was sequential hydrolysis with neutral protease for 2 h at a dose of 5100 U/g followed by trypsin at a dose of 950 U/g for another 2 h.The ACE inhibitory rate of final hydrolysis products was up to 72.21%.关键词
复合酶法/ACE抑制肽/Box-Behnken试验Key words
multienzymctic hydrolysis/ACE inhibitory peptides/Box-Behnken experimental design分类
生物科学引用本文复制引用
李景,王素英,张思憨,王园,李吴泽,林菘..复合酶法水解豆粕制备ACE抑制肽[J].食品科学,2012,33(9):163-166,4.基金项目
天津市科技支撑计划项目 （）
