食品科学2012，Vol.33Issue(9)：167-172,6.
酶解条浒苔蛋白制备ACE抑制肽
Preparation of ACE Inhibitory Peptides by Enzymatic Hydrolysis of Protein from Enteromorpha clathrata
摘要
Abstract
In order to exploit abundant proteins in the green algae Enteromorpha clathrata,Enteromorpha clathrata was enzymatically hydrolyzed and the angiotensin-converting enzyme（ACE） inhibitory activity of its hydrolysate was determined spectrometrically.Alcalase was found to be more suitable for the preparation of ACE inhibitory peptides from Enteromorpha clathrata proteins than alkaline protease and trypsin.Based on one-factor-at-a-time experiments,response surface methodology was employed to optimize the hydrolysis conditions.The optimal hydrolysis process conditions were solid/liquid ratio of 1：50,enzyme dose of 2982 U/g protein,hydrolysis temperature of 47.9 ℃,hydrolysis pH of 7.53 and hydrolysis duration of 90 min.Under these conditions,a hydrolysate with an ACE inhibitory IC50 of 0.66 mg/mL was obtained.The hydrolysate consisted of fractions with a MWCO（molecular weight cut-off） of 1-5 kD and the fraction with an average chain length of 16 exhibited the strongest ACE inhibitory activity.The fractions with molecular weight of lower than 10 kD showed resistance to gastrointestinal proteases in vitro.关键词
条浒苔/酶解/ACE抑制肽/AlcalaseKey words
Enteromorpha clathrata/enzymatic hydrolysis/angiotensin Ⅰ-converting enzyme(ACE) inhibitory peptide/alcalase分类
生物科学引用本文复制引用
盘赛昆,周鸣谦,姚东瑞,孙惠..酶解条浒苔蛋白制备ACE抑制肽[J].食品科学,2012,33(9):167-172,6.基金项目
江苏省海洋生物技术重点建设实验室开放基金项目 （）
