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响应面分析法优化酶提取甜茶茶多酚工艺

程雅芳 杨洋 温富雄 袁经权 缪剑华

食品科学2012,Vol.33Issue(10):10-15,6.
食品科学2012,Vol.33Issue(10):10-15,6.

响应面分析法优化酶提取甜茶茶多酚工艺

Optimization of Enzymatic Extraction for Tea Polyphenols from Sweet Tea by Response Surface Methodology

程雅芳 1杨洋 1温富雄 1袁经权 1缪剑华2

作者信息

  • 1. 广西大学生命科学与技术学院,广西南宁530004
  • 2. 广西药用植物园,广西南宁530023
  • 折叠

摘要

Abstract

The extraction process of polyphenols from sweet tea was explored using compound enzyme-assisted extraction.The compound enzymes were composed of cellulose and pectinase at a mixture ratio of 3:4.Based on response surface methodology,the optimal extraction conditions of enzyme-assisted were enzyme dosage of 0.6%,hydrolysis temperature of 45℃,extraction pH of 4.95,extraction time of 47.76 min,and solid-liquid ratio of 1:23.58.The ethanol extraction conditions were ethanol concentration of 40%(V/V),extraction time of 70 min,extraction temperature of 70℃ and solid-liquid radio of 1:28.Under the optimal extraction conditions,the yield of tea polyphenols was 133.2 mg/g.

关键词

甜茶/茶多酚//响应面分析

Key words

sweet tea/tea polyphenols: enzyme/response surface methodology

分类

轻工纺织

引用本文复制引用

程雅芳,杨洋,温富雄,袁经权,缪剑华..响应面分析法优化酶提取甜茶茶多酚工艺[J].食品科学,2012,33(10):10-15,6.

基金项目

广西科技厅科技攻关项目 ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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