食品科学2012，Vol.33Issue(10)：115-118,4.
软枣猕猴桃多聚半乳糖醛酸酶提取条件的优化
Optimization of Extraction Conditions for Polygalacturonase from Actinidia arguta
摘要
Abstract
Actinidia arguta was used as the experimental material to explore the optimal extraction conditions and enzymatic activity analysis conditions of polygalacturonase （PG）. The optimal extraction process parameters were explored by orthogonal tests through evaluating the effects of pH, ion concentration and DTT addition amount on extraction rate PG. The results indicated that the optimal extraction process condtions for PG were extraction pH of 5.5, acetate buffer concentration of 0.05 mol/L, NaC1 concentration of 0.1 mol/L and DTT concentration of 1 mmol/L. The optimal enzymatic activity analysis conditions were reaction temperature of 40 ℃ and reaction time of 90 min.关键词
软枣猕猴桃/多聚半乳糖醛酸酶/提取/活性分析Key words
Actinidia arguta/polygalacturonase (PG)/extraction/enzymatic activity analysis分类
轻工纺织引用本文复制引用
刘月,李书倩,张博,刘长江,辛广..软枣猕猴桃多聚半乳糖醛酸酶提取条件的优化[J].食品科学,2012,33(10):115-118,4.基金项目
国家公益性行业（农业）科研专项 （）
