食品科学2012，Vol.33Issue(10)：119-123,5.
两种来源酶多种方式水解牛骨蛋白
Dual-enzyme Hydrolysis of Bovine Bone Protein Using Different Methods
摘要
Abstract
Two proteases including Bacillus subtilis neutral protease and papain are used to hydrolyze bovine bone protein by five hydrolysis methods. The optimal dual-enzyme hydrolysis conditions of bone protein were explored for maximizing hydrolysis efficiency. The results indicated that synchronous hydrolysis of bone protein exhibited a higher hydrolysis effi- ciency when compared with the sequential hydrolysis by papain first and then Bacillus protease or Bacillus protease first and then papain. The optimal hydrolysis conditions were total enzyme amount of 7000 U/g at papain-neutral protease ratio of 1：1, oscillation hydrolysis time of 6 h, oscillation speed of 150 r/min, hydrolysis temperature of 51℃, hydrolysis pH of 7.05 and substrate concentration of 10%. Under the optimal hydrolysis conditions, the degree of hydrolysis was up to 27.54%.关键词
牛骨蛋白/水解/木瓜蛋白酶/枯草杆菌中性蛋白酶/水解度Key words
bone protein/hydrolysis/Bacillus protease/papain/degree of hydrolysis分类
轻工纺织引用本文复制引用
万婷婷,罗爱平,何光中,李丽,陈明..两种来源酶多种方式水解牛骨蛋白[J].食品科学,2012,33(10):119-123,5.基金项目
贵州省科技厅资助项目 （）
贵州省农业委员会资助项目 （）
贵州大学研究生创新基金项目 （）
