食品科学2012，Vol.33Issue(11)：121-125,5.
辅酶Q10高产菌株的诱变选育
Induced Mutation Breeding and Screening of a Coenzyme QlO-Producing Strain
摘要
Abstract
Rhizobium leguminosarum 1.1723 was mutagenized by means of LiCl treatment followed by UV irradiation or ultrasonic treatment alone to yield coenzyme Q10-producing strains. The results showed that 1 g/L LiCl could obviously increase the positive mutation rate and that ultrasonic treatment could change cell wall structure of Rhizobium leguminosarum. A mutant C40-05 with high productivity of coenzyme Q10 was obtained through preliminary screening with roxithromycin and secondary screening by means of shake flask cultivation. Its coenzyme Q10 yield was up to 1.198 mg/g, which revealed a 208% increase compared with the original stain. Only 2.67% reduction in coenzyme Q10 yield of the mutant was observed after the fifth passage. Therefore, the obtained mutant is a stable strain that deserves to be studied further.关键词
豌豆根瘤菌/辅酶Q10/紫外-LiCl/超声波/诱变Key words
Rhizobium leguminosarum/coenzyme Q10/UV-LiCl/ultrasonic/mutagenesis分类
轻工纺织引用本文复制引用
曹燕妮,岳田利,袁亚红,高振鹏..辅酶Q10高产菌株的诱变选育[J].食品科学,2012,33(11):121-125,5.基金项目
国家自然科学基金项目 （）
农业部“948”项目 （）
