食品科学2012，Vol.33Issue(11)：215-218,4.
大麦虫超氧化物歧化酶分离纯化及性质研究
Purification and Characterization of Superoxide Dismutase from Zophobas morio L. Larvae
张建新 1刘娜 1何桂梅 1郭倩1
作者信息
- 1. 西北农林科技大学食品科学与工程学院,陕西杨凌712100
- 折叠
摘要
Abstract
Objective： To explore the purification and properties of superoxidae dismutase（SOD） from Zophobas morio L.larvae.Methods： SOD were obtained from Zophobas morio L.larvae by salting-out,DEAE-Sepharose FF column chromatography and Sephadex G-75 column chromatography.SDS-PAGE was used to determine its molecular weight and atomic absorption spectrometer was used to identify it types.Results： SOD with highly specific activity was obtained.The purification factor was 68.54.The purified SOD was identified as Cu,Zn-SOD with molecular weight of 37.30 kD.It had an absorption peak at 278 nm and good stability.Its optimal temperature and pH were 40 ℃ and 6.0,respectively.Its high sensitivity to H2O2 and β-mercaptoethanol was found,while a mixture of CHCl3 and CH3CH2OH（3：5,V/V） had little effect on its activity.Conclusion： The SOD extracted from Zophobas morio L.larvae has high enzymatic activity,stability and application potential.关键词
大麦虫/超氧化物歧化酶/分离纯化/性质Key words
Zophobas morio L./SOD/purification/characterization分类
生物科学引用本文复制引用
张建新,刘娜,何桂梅,郭倩..大麦虫超氧化物歧化酶分离纯化及性质研究[J].食品科学,2012,33(11):215-218,4.
