食品科学2012，Vol.33Issue(12)：83-88,6.
响应面法优化油菜蜂花粉核苷类成分提取工艺
Optimization of Nucleosides Extraction from Rape Bee Pollen Using Response Surface Methodology
摘要
Abstract
Nucleosides extraction from rape bee pollen was optimized using response surface methodology. The appropriate ranges of extraction time, extraction temperature and material-to-liquid ratio were obtained by one-factor-at-a-time experiments for subsequent optimization using response surface analysis based on a three-variable, three-level central composite design. The optimal ranges of extraction time, extraction temperature and material-to-liquid ratio were 40-60 rain, 45 -65 ℃ and 1：60- 1：80 （g/mL）, respectively. The optimized extraction conditions were 53 min, 60 ℃, and 1：70 （g/mL）. Under these conditions, the extraction rate of nucleosides from rape bee pollen was （705.2 ± 0.006） mg/100 g.关键词
响应面法/核苷类成分/油菜蜂花粉/Box-Behnken/超声波提取/优化Key words
response surface methodology/nucleosides/rape bee pollen/Box-Behnken design/ultrasonic extractiomoptimization分类
农业科技引用本文复制引用
孙丽萍,黄兰,徐响,庞杰,何伟,穆雪峰,沈新锋..响应面法优化油菜蜂花粉核苷类成分提取工艺[J].食品科学,2012,33(12):83-88,6.基金项目
“十二五”国家科技支撑计划项目 （）
国家现代农业（蜂）产业技术体系建设专项 （）
科技部农业科技成果转化资金项目 （）
