食品科学2012，Vol.33Issue(12)：181-184,4.
反相高效液相色谱法测定果醋中的8种维生素
Determination of Eight Water-Soluble Vitamins in Fruit Vinegar by RP-HPLC
摘要
Abstract
A RP-HPLC gradient elution method was developed for the simultaneous determination of eight water-soluble vitamins, including ascorbic acid, thiamine, riboflavin, nicotinic acid, pyridoxine, cyanocobalamin, folic acid and nicotinamide in fruit vinegar. The effects of temperature, buffer solution concentration, pH value and gradient elution procedure on chromato- graphic separation were studied. The mobile phase was a mixture of 0.1 mol/L sodium acetate solution （pH 4.25） and methanol at a flow rate of 1.00 mL/min. The column temperature was set as 30 ~C. Baseline separation was achieved for 8 water-soluble vitamins within 14.2 min. The detection was carried out at 266 nm and 280 nm. The method offered excellent linearity over a range of 0.05-- 12.5 lag/mL for riboflavin and folic acid, 0.1 --25 ~t g/mL for ascorbic acid, thiamine, nicotinic acid and nicotinamide, 0.15 -- 37.5 /a g/mL for pyridoxine and cyanocobalamin, with correlation coefficients （r ~ 0.999）. The detection limits were in the range of 0.47--3.46 ng/mL This method has been successfully applied to assay vitamins in fruit vinegar. The average spike recovery varied from 92.4% to 105.2% with RSD in the range of 0.08% -- 1.54% （n =3）.关键词
反相高效液相色谱/水溶性维生素/果醋Key words
RP-HPLC/water-soluble vitamins/fruit vinegar分类
轻工纺织引用本文复制引用
宋萍萍,曹江平,刘书慧..反相高效液相色谱法测定果醋中的8种维生素[J].食品科学,2012,33(12):181-184,4.基金项目
西北农林科技大学引进人才专项 （）
