食品科学2012，Vol.33Issue(12)：210-214,5.
高效液相色谱-串联质谱法测定动物源食品中喹乙醇代谢物残留量
Determination of Olaquindox Metabolite Residues in Food Products of Animal Origin Using High Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） method was developed to determine the olaquindox metabolite marker 3-methyl-quinoxaline-2-carboxylic acid （MQCA） in food products of animal origin. Samples were homogenized, hydrolyzed with protease overnight, acidified by adding hydrochloric acid, centrifugated, filtrated, and then cleaned up and enriched using an Oasis MAX solid phase extraction cartridge. The chromatographic separation was performed on a Kinetex Clscolumn. The qualitative and quantitative analysis of MQCA was carried out using an HPLC-MS/MS system under positive ion multiple reaction monitoring （MRM） mode. The limits of quantification for MQCA in meats from 6 different animal species （eel, shrimp, chicken, pork, beef and porcine liver） were all 0.5μ g/kg. The average recoveries at four different spike levels： 0.5, 1.0, 4.0 pg/mg and 10.0 pg/kg were 60.7% -- 107% with relative standard deviation between 4.59% and 14.9% （n = 10）. This method was fast, sensitive and reproducible and all of its technical indexes could meet the requirements of domestic and international relevant standards. Therefore it is applicable for the determination and confirmation of MQCA residues in food products of animal origin.关键词
高效液相色谱-串联质谱法/喹乙醇/3-甲基喹喔啉-2-羧酸/动物源性食品Key words
liquid chromatography-tandemmass spectrometry (LC-MS/MS)/qlaquindox/3-methyl-quinoxaline-2-car-boxylic acid (MQCA)/food products of animal origin分类
农业科技引用本文复制引用
刘正才,杨方,余孔捷,林永辉,李立..高效液相色谱-串联质谱法测定动物源食品中喹乙醇代谢物残留量[J].食品科学,2012,33(12):210-214,5.基金项目
国家质检总局检验检疫行业标准制（修）定计划项目 （）
