中国食品学报2012,Vol.12Issue(6):16-21,6.
超滤法制备高抗菌抗氧化活性带鱼蛋白亚铁螯合肽(Fe-HPH)的工艺研究
Preparing Ferrous Chelating of Hairtail Protein Hydrolysate (Fe-HPH) with High Antibacterial and Antioxidant Activity by URrafiltration
摘要
Abstract
In order to obtain ferrous chelating of hairtail protein hydrolysate (Fe-HPH) with highly antibacterial and anlioxidant activity, the ultrafiltration membranes of Mw cut-off 10, 5, 3, 1 ku, were used to fractionate Fe- HPH. The antibacterial and antioxidant activity were compared before and after the fractionation. Then an orthogonal test was de- signed to optimize fractionation conditions. Results: The filtrate of Mw cut-off 3 ku ultrafiltration membrane has the high antibacterial and antioxidant activity, and gives an antibacterial activity above 91.3% and DPPH radical scavenging activities above 84.6% with the optimum fractionation conditions as concentration 30%, pH 6.5, and temperature 35 ℃.关键词
带鱼蛋白亚铁螯合肽( Fe-HPH)/超滤/分级分离/抗菌/抗氧化Key words
ferrous chelating of hairtail protein hydrolysate(Fe-HPH)/ ultrafiltration/ fractionation/ antibacterial activity/ antioxidant activity引用本文复制引用
林慧敏,邓尚贵,庞杰,张宾..超滤法制备高抗菌抗氧化活性带鱼蛋白亚铁螯合肽(Fe-HPH)的工艺研究[J].中国食品学报,2012,12(6):16-21,6.基金项目
国家自然科学基金项目(31071628) (31071628)
国家国际科技合作项目(2010DFB34220) (2010DFB34220)
国家科技支撑项目(2009BADB9B09-1) (2009BADB9B09-1)