中国食品学报2012，Vol.12Issue(6)：191-197,7.
毛细管电泳-电化学发光法分离检测鱿鱼丝中的组胺
Determination and Separation of Histamine in Shredded Squid by Capillary Electrophoresis with Electrochemical Luminescence Detection
摘要
Abstract
In this research, a novel method is built for determination and separation of histamine in Shredded squid and its products by capillary electrophoresis with an electrochemical luminescence detection (CE-ECL) based on tris (2, 2'-bipyridyl)ruthenium(II) [Ru(bpy)32+]. This paper discussed the influence of several experiment parameters such as detection cell conditions (detection potential, concentration of tris(2,2'-bipyridyl)ruthenium(II) and pH of detection cell), injection parameters (injection voltage and injection time) and running parmeters( separation voltage, concentration and pH of running buffer) were explored. Under the optimal conditions, such as detection potential at 1.15 V, 5 mmol/L Ru(bpy)32+ (pH 7.0), sample injection at 8 s×10 kV, separation voltage at 18 kV, 50 mmol/L of phosphate buffer at pH 3.4. under its optimal conditions, the ECL intensity was linear with concentration of histamine dihydrochloride, the linear equation for I= 1.42c- 30.83, correlation coefficient r = 0.9981 (n = 6) with detection limit(S/W=3) of 0.468 μmol/L. The method used to determinate histamine in the squid silk samples, the relative standard deviation(RSD) of peak height and migration time were 0.43% and 0.29%, recovery is 100.32%~106.55%. This method is suitable for the rapid detection of food, due to its rapid analysis, good separation, high sensitivity, good reproducibility.关键词
毛细管电泳/电致化学发光/三联吡啶钌/组胺Key words
capillary electrophoresis/ electrochemiluminescence/ Ru(bpy)32+/ histamine引用本文复制引用
钱勇强,沈俊炳,王力..毛细管电泳-电化学发光法分离检测鱿鱼丝中的组胺[J].中国食品学报,2012,12(6):191-197,7.基金项目
国家自然科学基金项目(20871054) （20871054）
福建省自然科学基金高校专项基金项目(JK2011027) （JK2011027）
