TaqMan探针与SYBR Green实时定量PCR法检测转基因植物外源基因拷贝数的差异分析OA北大核心CSCDCSTPCD
Variance analysis between TaqMan probe and SYBR Green real-time quantitative PCR for determining the copy number of transgene in transformed plants
探讨TaqMan探针与SYBR Green实时定量PCR2种方法检测转基因植物外源基因的拷贝数方法的差异.以12株T0转基因水稻为材料,分别用TaqMan与SYBR Green实时定量PCR法检测其拷贝数,然后用SAS 9.1软件对2种方法的结果进行t检验分析.通过SAS对2组数据的t检验分析,在内参基因扩增效率与目的基因扩增效率接近时,SYBR Green与TaqMan探针法的结果接近,差异不显著；当内参基因与目的基因扩增效率差异明显时,SYB…查看全部>>
To explore the differences between TaqMan probe and SYBR Green real-time quantitative PCR technique for estimating the transgene copy number in a transgenic plant, we used TaqMan probe and SYBR Green real-time quantitative PCR technique to determine the copy number of twelve transgenic rice, and then used the software SAS 9.1 to analyze the results with the two methods. As a result, the t-test analysis showed that when the amplification efficiency of the end…查看全部>>
李敏;汪洋;张银萍;李娟;陈学平
安徽农业大学生命科学学院,合肥230036中国科技大学化学与材料科学学院,合肥230026合肥工业大学生物与食品工程学院,合肥230009中国科技大学化学与材料科学学院,合肥230026安徽农业大学生命科学学院,合肥230036
生物科学
转基因水稻TaqMan探针SYBR Green拷贝数t检验
transgenic rice TaqMan probe SYBR green copy number t-test
《安徽农业大学学报》 2012 (4)
568-570,3
科技部重大转基因专项(2009ZX08009-063B)资助.
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