滤纸壳聚糖膜固定乙酰胆碱酯酶的研究OA
Study on Immobilization of Acetylcholinesterase with Filter Paper Coated with Chitosan
[目的]探索滤纸壳聚糖膜固定乙酰胆碱酯酶( AChE)的最佳条件.[方法]以滤纸壳聚糖膜为我体,戊二醛为交联剂,牛血清白蛋白( BSA)为保护剂,进行AChE的固定.并对固定化酶的理化性质进行研究.[结果]固定AChE的最佳条件为将50μl150 U/mlAChE液,50 μl 5％(V/V)戊二醛溶液,100μl1％(W/W) BSA,pH 8.0的0.2 mol/L的PBS缓冲液配制成1 ml酶液,滤纸壳聚糖膜浸于该酶液4℃固定8h.固定化酶…查看全部>>
[Objective] The aim was to explore the optimum immobilized conditions of acetylcholinesterase on filter paper coated with chitosan. [Method] Acetylcholinesterase was immobilized on filter paper coated with chitosan using glutaraldehyde as cross-linking reagent, bovine serum albumin as protective agent. And the physical and chemical properties of the immobilized enzyme were studied. [Result] The optimal immobilized conditions were as follows: 1 ml solution wa…查看全部>>
郭尽力;姜爱莉;于贞;郑舒文
烟台大学生命科学学院,山东烟台264005烟台大学生命科学学院,山东烟台264005烟台大学生命科学学院,山东烟台264005烟台大学生命科学学院,山东烟台264005
农业科技
滤纸壳聚糖膜乙酰胆碱酯酶固定化农药残留
Filter paper coated with ehitosanAcetylcholinesterase ImmobilizationPesticide residue
《安徽农业科学》 2012 (23)
11653-11655,3
山东省自然科学基金项目(ZR2011DL008,Y2008D48).
评论