茶叶科学2012，Vol.32Issue(4)：289-296,8.
茶叶黄酮类物质的双水相系统纯化及抗氧化研究
Study on Extraction by Aqueous Two-phase System and Anti-oxidation of Flavonoids from Tea
摘要
Abstract
To explore antioxidation capability and the extraction mechanism of the separation-purification of flavonoids from tea in aqueous two-phase systems, flavonoids from tea was taken as an index, to optimize the conditions for the extraction of flavonoids from tea, the effect of each factor such as weight and amounts of PEG, kinds and amounts of salt, temperature, pH have been studied by single factor and response surface methodology. Based on the single factor experiments, a three-factor (the amount of Na2SO4, PEG600, MgCl2) and three-level experiment design has been developed by Box-Behnken central composite design method. The antioxidant activity of different concentration flavonoids were assessed through reducing power assay, Scavenging effects on DPPH radicals and results were compared to reference antioxidant BHT and Vc. The result showed that the optimum conditions as follows, pH 5, the extracting temperature 30?, the amount of 5 mL Na2SO4 15%, the amount of 6 g PEG600, the amount of MgCl2 2.9%, the extraction field 96.31%. The two aqueous phase system for fiavonoid extraction is easy to get good reproductivity, and applied in mass production. The flavonoids from tea showed strong antioxidant activity. The flavonoids from tea are a potential product of natural safe and effective antioxidant.关键词
茶叶/黄酮/双水相体系/响应面法/抗氧化Key words
tea/ fiavnoid/ aqueous two-phase system/ response surface analysis/ anti-oxidation分类
轻工纺织引用本文复制引用
吴祥庭,王爱银,周化斌,李国兴,徐姗姗..茶叶黄酮类物质的双水相系统纯化及抗氧化研究[J].茶叶科学,2012,32(4):289-296,8.基金项目
浙江省科技厅项目(No.2010C32005) （No.2010C32005）
