茶叶科学2012，Vol.32Issue(4)：313-318,6.
茶叶中EGCG3"Me和EGCG4"Me的分离制备与鉴定
Isolation and Identification of EGCG3"Me and EGCG4"Me from Tea
摘要
Abstract
A method was established to isolate EGCG3"Me and EGCG 4"Me by using column chromatography and preparative high performance liquid chromatography. Crude extracts was obtained from tea leaves by using ethyl acetate and water as solvent .Then the concentracted crude extract was separated on a HP-20 column. The Fr-1 fraction was obtained from the eluate of 80% ethanol and subjected to a preparative high performance liquid chromatography for isolation of target components by using water-acetonitrile as mobile phase. Two compounds were identified with ultraviolet absorbance(UV), nuclear magnetic resonance(NMR), mass spectrometry(MS). They were EGCG3"Me and EGCG4"Me.The purity of them was more than 98%.关键词
EGCG3"Me/EGCG4"Me/分离制备/茶叶Key words
EGCG3"Me/ EGCG4"Me/ isolation and preparation/ tea分类
轻工纺织引用本文复制引用
李银花,张盛,黄建安,刘仲华..茶叶中EGCG3"Me和EGCG4"Me的分离制备与鉴定[J].茶叶科学,2012,32(4):313-318,6.基金项目
国家自然科学基金(31100501)和湖南农业大学青年基金(11QN10) （31100501）
