现代食品科技2012，Vol.28Issue(7)：758-763,767,7.
婴儿粪便中乳酸菌的分离鉴定及其对小鼠腹泻的治疗作用
Isolation and Identification of the Lactic Acid Bacteria from Infant Faeces and their Therapeutical Effect on Diarrhea of Mice
摘要
Abstract
Six Gram-positive bacteria with strong acidogenicity were separated and purified from fresh feces of healthy babies, and their bacteriostasis in vitrv were tested. Three strains with high inhibition effect on diarrhoeic Escherichia coli (078, 088 and 0149) were screened. The results of biochemical identification and 16S rRNA comparison analysis showed that alt the three screened strains were Laetobacillus casei. The challenged test in mice and chickens by one strain with strongest inhibition on pathogenic E. Coli showed that the strain was safe, nontoxic and appropriately improved the animal feed conversion rate, which can used for prevention and treatment of diarrhea effect.关键词
腹泻/干酪乳杆菌/抑菌/分离/纯化/生化鉴定Key words
diarrhea/ Lactobacillus casei/ bacteriostasis/ separation/ purification/ biochemical identification引用本文复制引用
郑明英,周海泳,李适云,胡文锋,刘秉杰,杨益衡,陈永泉..婴儿粪便中乳酸菌的分离鉴定及其对小鼠腹泻的治疗作用[J].现代食品科技,2012,28(7):758-763,767,7.基金项目
广东省科技厅省部产学研结合项目(2011B090400221):广东省教育部产学研结合项目(2009B090300106,2010809400038):广东省科技厅农业攻关项目(20106020412001) （2011B090400221）
