现代食品科技2012，Vol.28Issue(7)：764-767,4.
巴氏杜氏藻ξ-胡萝卜素脱氢酶基因的克隆与分析
Clone and Analysis ζ- Carotene Desaturase of Dunaliella bardawil
摘要
Abstract
ζ-Carotene desaturase (ZDS) is one of the key enzymes in the carotenoid biosynthetic pathway. According to the full-length sequence of the ZDS cDNA in D. Bardawil that our laboratory has obtained before, the coding region sequence of ZDS were isolated by the approach of PCR and then flanking sequence of the coding region were amplified by the method of genome walking, including promoter and terminator of ZDS, followed by analysis of these sequences by bioinformarics tools. The whole sequence of ZDS was 11896 bp. It contained 6435 bp coding region (from ATG to TAA), 4091 bp upstream of the coding region, and 1370 downstream of the coding region. Blastn the coding region and ORF of ZDS showed that there were 12 exons and 11 introns in coding region. The total length of the introns was 4086 bp, about 2.68 fold of the total length of exons (1749bp). Each intron started with GT and end with AG, belonging to the most common style of exon. By analyzing the promoter of ZDS gene, we found that there are many transcription factor binding sites, including "GAGA box", ASF1, related with plant light regulation; "ACGT box", required for etiolation-induced expression of early responsive to dehydration.关键词
巴氏藻/ξ-胡萝卜素脱氢酶/编码区/启动子/生物信息学Key words
Dunaliella bardawil/ ζ-camtene desaturase/ coding region/ promoter, bioinformarics引用本文复制引用
肖岚,劳永民,姜建国..巴氏杜氏藻ξ-胡萝卜素脱氢酶基因的克隆与分析[J].现代食品科技,2012,28(7):764-767,4.基金项目
国家自然科学基金项目(31171631) （31171631）
