现代食品科技2012，Vol.28Issue(7)：882-885,4.
简易顶空气相法测定小鼠血液中的乙醇含量
Detection of Alcohol Concentration in Rat Blood by a Simple Headspace Gas Chromatography
摘要
Abstract
The purpose of this study was to establish an ultra-sensitive and handy headspace gas chromatographic for the determination of alcohol in rat blood by gas chromatograph. The blood samples containing alcohol and tert-butanol (internal standard, IS) were heated in a water bath. The headspace vapor was drawn into a syringe and injected into a GC port and the experimental conditions of headspace and GC were optimized by taking the peak areas as index. The results showed that the samples heated at 70 ℃ for 15 min had a steady peak area and good vapor-liquid equilibrium. Under the optimized conditions, the target peak and the internal standard peak were clearly separated. In the range of 0.05-12 mg/mL, the linear relation between peak area and concentration of the alcohol was found. RSD was 1.63% by testing the standard solution for seven times. The detection limit was 0.005 mg/mL and the reclaim ratio was 93.4%~103.2% (RSD≤41%). In conclusion, this method was simple, flexible and effective to detect the alcohol concentration in rats' blood and can be used widely.关键词
气相色谱/血液乙醇浓度/顶空法/内标法Key words
gas chromatography/ blood alcohol concentration/ headspace gas chromatographic/ internal standard引用本文复制引用
左光扬,章超桦,高加龙,秦小明,曹文红,刘书成..简易顶空气相法测定小鼠血液中的乙醇含量[J].现代食品科技,2012,28(7):882-885,4.基金项目
国家现代农业产业技术体系(CARS-48) （CARS-48）
