现代食品科技2012，Vol.28Issue(8)：1049-1053,1087,6.
一株产L-赖氨酸菌株发酵培养基的响应面优化
Optimization of the Fermentation Medium of a L-lysine-producing Strain by Response Surface Methodology
周旭波 1庄楚周1
作者信息
- 1. 广东环西生物科技股份有限公司,广东揭阳515343
- 折叠
摘要
Abstract
The fermentation medium of a L-lysine producing strain Brevibacterium flavum HXL109 to improve the L-lysine production was optimizd. With the software Design Expert, a response surface model was established using tire Box-Behnken design(BBD). The effects of glucose, (NH4)2SO4, soybean cake hydrorysate, KH2PO4·3H2O, MgSO4·7H2O, FeSO4·7H2O and MnSO4·H2O on L-lysine yield were analyzed. The results showed that the optimum conditions for HXL109 to produce L-lysine were as follows: glucose 89.48g/L, soybean cake hydrolysate 30.77g/L, (NH4)2SO4 20.89g/L and KH2PO4·3H2O 4.5g/L. Under these conditions, the result of confirmatory experiment (142.65g/L) was in good agreement with the predicted value (143.67g/L). A comparison study showed that the established regression model had good effectiveness in optimizing the fermentation medium of a L-lysine producing strain.关键词
L-赖氨酸/响应面/对比实验Key words
L-lysine/ RSM/ comparing experiment引用本文复制引用
周旭波,庄楚周..一株产L-赖氨酸菌株发酵培养基的响应面优化[J].现代食品科技,2012,28(8):1049-1053,1087,6.
