现代食品科技2012，Vol.28Issue(8)：1054-1057,4.
蛹虫草多糖的纯化及其分子量的测定
Isolation, Purification and Molecular Weight Determination of Polysaccharides from Cordyceps militaris
摘要
Abstract
Crude polysaccharides (CP) were extracted from dried fruits of Contyceps militaris (L.ex Fr) Link, through sequential steps of hot water extraction, ethanol precipitation and zinc sulfate deproteination. It was fractionated by membrane separation, purified by DEAE-cellulose-52 exchange chromatography and further purification by Sephadex G-100 gel filtration chromatography. Their molecular weights were determined using high performance gel-permeation chromatography (HPGPC) in order to characterize molecular weight distribution. The results showed that CP2-c2-s2 was a homogeneous polysaccharide with average molecular weight of 20200 Da, which could be obtained through aforementioned isolation and purification.关键词
蛹虫草多糖/分离纯化/凝胶渗透色谱Key words
Confyceps militaris polysaccharides/ purification/ gel permeation chromatography引用本文复制引用
黄奕诚,陈雪香,贺丽苹,陈淑敏,华洋林,陈奇,曹庸..蛹虫草多糖的纯化及其分子量的测定[J].现代食品科技,2012,28(8):1054-1057,4.基金项目
复合活性多糖的提取分离技术及评价研究(PPD/11/05/005) （PPD/11/05/005）
