食品工业科技2012，Vol.33Issue(10)：212-215,4.
Alcalase蛋白酶酶解高温豆粕制备水溶性大豆多肽
Preparation of water soluble peptides hydrolyzing high-temperature soybean meal by Acalase protease
摘要
Abstract
Alcalase protease was used to hydrolyze the high-temperature soybean meal to improve the nitrogen solubility index（NSI）.The optimum conditions of enzymatic hydrolysis were as follows：Enzyme quantity 1750U/g,substrate concentration 4%（w/w）,temperature 60℃,pH9.0,and time 3h.Under the optimum conditions,the NSI value was 62.97% which was higher than before hydrolysis（47.87%）.SDS-PAGE analysis showed that Alcalase protease could quickly hydrolyze soy protein.The α subunit,α＇ subunit and β subunit of 7S and acidic subunits of 11S were completely degraded after hydrolysis of 1h.After 3h,basic subunits of 11S disappeared and the molecular weight of the peptides were almost under 20ku.Compared with SPI hydrolysate,the bitterness of high temperature hydrolysate was low.关键词
Alcalase蛋白酶/高温豆粕/氮溶指数/酶促降解/苦味Key words
Alcalase protease/high-temperature soybean meal/nitrogen solubility index/enzymatic degradation/bitterness分类
轻工纺织引用本文复制引用
张静,李理..Alcalase蛋白酶酶解高温豆粕制备水溶性大豆多肽[J].食品工业科技,2012,33(10):212-215,4.基金项目
国家自然科学基金 （）
