食品工业科技2012，Vol.33Issue(10)：223-225,3.
固定化葡萄糖异构酶活化条件对其酶活的影响
Influence of activation conditions on the enzyme activity of immobilized glucose isomerase
胡弢 1周雪艳 1赵国群1
作者信息
- 1. 河北科技大学生物科学与工程学院,河北石家庄050018
- 折叠
摘要
Abstract
Activation was need before immobilized glucose isomerase was used in order to make it have the best catalytic ability.The influences of activation conditions on the enzyme activity of GENSWEETTM IGI-SA immobilized glucose isomerase were studied including concentration of glucose syrup,temperature,pH,activation time and metal ions.The optimal activation condition was as follows：Glucose syrup 60%,temperature 55℃,pH 7.5 and activation time 4h.Under this condition,the enzyme activity of immobilized glucose isomerase was 815U/g,which was 40% higher than one under normal activation condition.Mg2＋,Co2＋,Mn2＋and Zn2＋were not necessary to be added into glucose syrup when immobilized glucose isomerase was activated.关键词
活化/果葡糖浆/固定化葡萄糖异构酶Key words
activation/high fructose syrup/immobilized glucose isomerase分类
轻工纺织引用本文复制引用
胡弢,周雪艳,赵国群..固定化葡萄糖异构酶活化条件对其酶活的影响[J].食品工业科技,2012,33(10):223-225,3.
