食品工业科技2012，Vol.33Issue(10)：243-246,4.
花生非淀粉多糖的纯化技术研究
Purification technology research of peanut non-starch polysaccharides
摘要
Abstract
Peanut polysaccharide was extracted from cold-pressed peanut meal by high-temperature water.The orthogonal array design was applied to optimize the process of removing starch by enzymatic hydrolysis,and peanut polysaccharide was isolated and purified by DEAE-52 cellulose ion-exchange column chromatography.Under the optimum condition,the purity of peanut polysaccharide was improved significantly after enzymatic hydrolysis using thermostable α-amylase and glucoamylase.Neutral polysaccharide PPS-1 and acidic polysaccharide PPS-2 were obtained through DEAE-52 column chromatography,and the content of these two kinds of polysaccharides was 17.79% and 66.93% respectively.UV spectral analysis indicated both PPS-1 and PPS-2 didn＇t contain nucleic acids,and that PPS-1 contained little protein.关键词
花生多糖/酶解淀粉/正交实验/分离纯化Key words
peanut polysaccharide/enzymatic hydrolysis starch/orthogonal array design/isolation and purification分类
轻工纺织引用本文复制引用
涂宗财,王蓓蕾,王辉,许丽敏,方杨..花生非淀粉多糖的纯化技术研究[J].食品工业科技,2012,33(10):243-246,4.基金项目
国家自然科学基金项目 （）
江西省“赣鄱英才555工程” （）
国家大学生创新性实验计划项目 （）
