食品工业科技2012，Vol.33Issue(16)：221-223,245,4.
响应面法优化溶藻弧菌的培养条件
Optimization of cultivation conditions of vibrio alginolyticus by response surface methodology
摘要
Abstract
According to singe-factor experiments,three levels factors including the pH,temperature and salinity were selected to evaluate the optimum culture condition of Vibrio alginolyticus. Based on Box-Behnken central composite design principle of the Response Surface Methodology（RSM）,the OD600 was chosen as response value to determine the range of factors and levels. With the results ,the optimized conditions of cultivation were as follows：pH7.62,temperature 34.55℃ and salinity 3.22% ,respectively. During the germination significant interactions between pH and temperature,as well as temperature and salinity were found,关键词
溶藻弧菌/Box-Behnken/响应面/培养条件Key words
Vibrio alginolyticus/Box-Behnken/response surface methodology(RSM)/culture condition分类
轻工纺织引用本文复制引用
张晓艳,宁喜斌..响应面法优化溶藻弧菌的培养条件[J].食品工业科技,2012,33(16):221-223,245,4.基金项目
上海市科委工程中心建设 （）
上海市教育委员会重点学科建设项目资助 （）
