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粗壮脉纹孢菌发酵稻谷加工副产物生产类胡萝卜素培养基的优化

刘志刚 李静 张丽美 邓泽元 范亚苇

食品工业科技2012,Vol.33Issue(16):277-280,4.
食品工业科技2012,Vol.33Issue(16):277-280,4.

粗壮脉纹孢菌发酵稻谷加工副产物生产类胡萝卜素培养基的优化

Optimization of culture medium composed of rice by-products for carotenoid production by fermentation of Neurospora crassa

刘志刚 1李静 1张丽美 1邓泽元 1范亚苇1

作者信息

  • 1. 南昌大学,食品科学与技术国家重点实验室,高等研究院,江西南昌330047
  • 折叠

摘要

Abstract

The culture medium composed of rice by-products(rice chaff and defatted rice bran) for carotenoid production by Neurospora crassa was optimized through statistical experiment design and data analysis. The defatted rice bran, (NH4)2SO4, KH2PO4,MgSO4,MnSO4 and CaCI2 were evaluated as factors affecting carotenoid biosynthesis by Neurospora crassa,and the significant factors were screened by a Plackett-Burman design. Then the culture medium was optimized by response surface methodology. Results indicated that defatted rice bran and (NH4)zSO4 were the significant factors on carotenoid production. The optimal culture medium consisted of 64.58% defatted rice bran,33.85% rice chaff and 1.57% (NH4)~O4 at which the yield of carotenoids was gotten up to 366mg/kg culture using the optimized fermentation parameters.

关键词

类胡萝卜素/脱脂米糠/砻糠/粗壮脉纹孢菌/响应面法

Key words

carotenoid/defatted rice bran/rice chaff/Neurospora crassa/response surface methodology

分类

轻工纺织

引用本文复制引用

刘志刚,李静,张丽美,邓泽元,范亚苇..粗壮脉纹孢菌发酵稻谷加工副产物生产类胡萝卜素培养基的优化[J].食品工业科技,2012,33(16):277-280,4.

基金项目

食品科学与技术国家重点实验室基金项目 ()

江西省科技厅项目 ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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