食品工业科技2012，Vol.33Issue(16)：358-363,6.
DNA的滚环扩增技术研究进展
Research progress of rolling circle amplification of DNA
摘要
Abstract
As an isothermal DNA amplification technology,the well-known RCA （rolling circle amplification） has gained wide attention for its simplicity and high efficiency. Generally,forming specifically the circular DNA through hybridizing to the target molecule is the key point to realize specific detection. RCA has been extensively applied to immunoassay,in situ detection,SNP investigation and microarray assay. This review would introduce the principle,technological development,applicable prospect and the present problems of RCA.关键词
核酸检测/恒温核酸扩增/滚环扩增/锁式探针/核酸复制Key words
nucleic acid detection/isothermal nucleic acid amplification/RCA/padlock probe/nucleic acidreplication分类
生物科学引用本文复制引用
王晓亮,王星宇,梁长城,董平,梁兴国..DNA的滚环扩增技术研究进展[J].食品工业科技,2012,33(16):358-363,6.基金项目
“国家青年千人计划” （）
“长江学者和创新团队发展计划” （）
“山东省万人计划” （）
