食品工业科技2012，Vol.33Issue(17)：147-150,4.
红毛藻R-藻红蛋白的高效制备以及抗体标记与检测
Preparation of R-phycoerythrin from red alga and its application for antibody labeling and detection
摘要
Abstract
R-phycoerythrin（R-PF） was highly purified from red alga（ Bangia fusco-purpurea）by ammonium sulfate fractionation and DEAE- Sepharose anion- exchange column chromatography, the purity rate （A565/A280 ） of R- PE was over 6.0.SDS-PAGE demonstrated that relative molecular masses of R-PE subunits were about 19 and 21ku, respectively.Optimum concentration of heterobifunctional reagent N-succinimidyl-3-2-pyridyldithio propionate （SPDP）was used to polymerize goat anti-mouse IgG antibody and the cross-linking was identified using SDS-PAGE and fluorescence analysis.R-PE was successfully crosslinked with goat anti-mouse I gG as secondary antibody.Detection of major allergen parvalbumin in fish using R-PE labelled IgG were carried out by Dot blot and Western blot together with mouse anti-silver carp parvalbumin as primary antibody.The results demonstrated that R-PE labelled IgG could effectively detect the existence of parvalbumin and revealed good immunological specificity.The application of R-phycoerythrin as fluorescent probe is expected to shorten the detection time of immune hybridization ,and simplify the operation processes.关键词
R-藻红蛋白/荧光探针/交联/免疫检测Key words
R-phycoerythrin/fluorescent probes/crosslinking/immunization detection分类
轻工纺织引用本文复制引用
刘婷,沈建东,刘光明,蓝伟光,曹敏杰..红毛藻R-藻红蛋白的高效制备以及抗体标记与检测[J].食品工业科技,2012,33(17):147-150,4.基金项目
福建省重大科研专项 （）
福建省自然科学基金（2010J01044）. （）
