食品工业科技2012，Vol.33Issue(17)：154-158,5.
重组海藻糖合酶工程菌高密度发酵条件的研究
High cell density fermentation condition of trehalose synthase genetic engineering bacteria
摘要
Abstract
Object ：To obtain the high dense fermentation procedure of trehalose synthase（Tres）genetic engineering bacteria previously construct. Methods： Studied the influence of medium, pH, ferment mode for incubation and induction on the growth of recombinant genetic engineering bacterial and expression of target protein in a 10L auto control biostat fermentator, and explored the genetic stability of recombinant plasmid. Result： The highdensity culture medium of trehalose synthase genetic engineering bacteria was 2YT ＋ 0.2% glucose,the optimum pH was 7.0,the ferment mode was fed-batch.After the recombinant genetic engineering bacterial strain cultured in 10L high-density fermentation,the density of bacteria reached 50.78g/L,the enzyme activity reached 3.197U/mL.The constructed recombinant plasmid was inherited steadily in host bacteria.Conclusion ：The high-density fermentation condition of trehalose synthase genetic engineering bacteria was optimized. It laid a foundation of largescale production of trehalose.关键词
海藻糖合酶/基因工程菌/高密度发酵Key words
trehalose synthase(Tres)/genetic engineering bacteria/highdensity fermentation分类
轻工纺织引用本文复制引用
陈颖,杨丽维,齐欣,陈晓云,李明春,唐柳,张峻..重组海藻糖合酶工程菌高密度发酵条件的研究[J].食品工业科技,2012,33(17):154-158,5.基金项目
天津市应用基础及前沿技术研究计划 （）
国家自然科学基金（21076162）. （）
