食品工业科技2012，Vol.33Issue(17)：159-162,4.
基于PCR方法敲除黄酒酵母精氨酸酶基因的工程菌构建
A PCR-based method for construction of a genetic engineering Chinese rice wine yeast by disrupting the CARl gene
摘要
Abstract
Gene targeting cassettes were synthesized by long flanking homology PCR（LFHPCR） ,a new cloning free strategy to delete genes,and were transformed into yeast.A engineering Chinese rice yeast was constructed by disruption of CARl gene. Compared to the parental wild type, the engineering strain exhibited reduced production of EC and urea in Chinese rice wine.They were reduced by 72% and 38%, respectively. It was also suggested that Chinese rice wine brewed with the recombinant yeast does not affect overall fermentation profiles.关键词
氨基甲酸乙酯/黄酒/酵母/精氨酸酶基因/长侧翼同源PCRKey words
ethyl carbamate: Chinese rice wine: yeast : CARl : LFH- PCR分类
生物科学引用本文复制引用
赵然然,陆健,谢广发..基于PCR方法敲除黄酒酵母精氨酸酶基因的工程菌构建[J].食品工业科技,2012,33(17):159-162,4.基金项目
绍兴市科技计划项目 （）
教育部新世纪优秀人才支持计划（NCET-08-0790）： （）
