食品工业科技2012，Vol.33Issue(17)：215-219,5.
二次通用旋转组合设计法优化酶法提取茶叶籽蛋白工艺
Optimization of technological conditions for enzymatic extraction of tea seeds protein with quadratic regression rotational composite design
冯磊 1麻成金 2黄群 2彭忠瑾 1黄伟 1向勇平3
作者信息
- 1. 吉首大学食品科学研究所,湖南吉首416000
- 2. 吉首大学食品科学研究所,湖南吉首416000 吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000
- 3. 湖南省古丈县科技局,湖南吉首416300
- 折叠
摘要
Abstract
The technological conditions for enzymatic extraction of tea seeds protein was optimized by using alkaline proteinase with quadratic regression rotational composite design.On the base of single factors experiments, main factors that affecting the extraction efficiency, such as dosage of Alkali protease, pH value, enzymatic extraction time and temperature, were selected. And the regression mathematics model was established with extraction efficiency as criteria. With variance analysis of experiment results and optimization of mathematics model, the optimum conditions for enzymatic extraction of tea seeds protein were supposed to be follows：solid-liquid ratio 1：25,dosage of alkali protease 200U/g, pill0.5, enzymatic extraction time 55min, temperature 45℃. In these optimum conditions,extraction efficiency of tea seeds protein could be reached to 75.83%.关键词
茶叶籽蛋白/酶法提取/二次通用旋转组合设计/工艺优化Key words
tea seed protein enzymatic extraction quadratic regression rotational composite design/processoptimization分类
轻工纺织引用本文复制引用
冯磊,麻成金,黄群,彭忠瑾,黄伟,向勇平..二次通用旋转组合设计法优化酶法提取茶叶籽蛋白工艺[J].食品工业科技,2012,33(17):215-219,5.
