食品工业科技2012，Vol.33Issue(17)：325-329,5.
恩诺沙星化学发光免疫分析试剂盒的研制
Development of an indirect competitive chemiluminescence enzyme immunoassay kit for detecting of enrofloxacin residue
摘要
Abstract
A HRP-Luminol-H202 system based indirect competitive chemiluminescence enzyme immunoassay （CLEIA） kit for determination of enrofloxacin（ENR） residues was developed.The CLEIA conditions were optimized. The optimal concentration of coating antigen was 0.5ng/mL and the antibody dilution was 1 ：2000.Using PBST as assay buffer,the incubation time for the primary antibody was 30rain. Under the optimal conditions, the standard curve was developed.The regression equation for ENR was y = -22842x ＋ !72936 with a linear detection ranging from 0.02.4 to 2.76ng/mL. The IC50 was 0.106ng/mLThe averaged recoveries of ENR from spiked honey, milk, shrimp and fish samples were 96%- 116% ,90% -97% ,92% - 112% and 86%- 112%, respectively.The coefficient of variation of intra-assay and inter-assay was less than 15%.The developed kit can be stored at 4℃ for 180 davs.关键词
恩诺沙星/化学发光免疫分析/试剂盒Key words
enrofloxacin/chemiluminescence enzyme immunoassay/kit分类
轻工纺织引用本文复制引用
刘邓,丁飞,侯瑞霞,徐振林,孙远明,杨金易..恩诺沙星化学发光免疫分析试剂盒的研制[J].食品工业科技,2012,33(17):325-329,5.基金项目
广东省教育部产学研结合项目（2010A090200084,2011A090200029,20098090300409）. （）
