食品科学2012，Vol.33Issue(13)：183-186,4.
紫外和He—Ne激光复合诱变获得高产γ-聚谷氨酸产生菌的研究
Screening of a High-Yield ofγ -Ployglutamic Acid-Producing Strain by Means of Both UV Light and He-Ne Laser
摘要
Abstract
Bacillus licheniformis BCPG006 was mutagenized by means of both UV and He-Ne laser. A mutant strain named as Bacillus licheniformis BCPG078 having a high ability to produce γ-polyglutamic acid （F-PGA）, was obtained by resistance screening. The γ-PGA yield of the mutant strain was 16 g/L during 72 h of shake flask culture at 37 ℃, initial pH 7 and 200 r/min, which was 2.67-fold higher than that obtained from the original strain （6 g/L）. These results suggest that the mutant strain has strong genetic stability. Meanwhile, combination of UV and He-Ne laser provides an effective method for the screening of γ-PGA- producing strains.关键词
γ-聚谷氨酸/复合诱变/抗性平板/发酵条件Key words
γ-PGA/compound mutation/resistant plate/fermentation condition分类
生物科学引用本文复制引用
乔长晟,李雪,兰铃方,陈笑,马正旺,李政..紫外和He—Ne激光复合诱变获得高产γ-聚谷氨酸产生菌的研究[J].食品科学,2012,33(13):183-186,4.基金项目
天津市科技支撑重点计划项目 （）
