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李果实贮藏期间4株病原真菌的分离、鉴定及碳源代谢指纹图谱分析

王友升 陈玉娟 张燕

食品科学2012,Vol.33Issue(13):235-239,5.
食品科学2012,Vol.33Issue(13):235-239,5.

李果实贮藏期间4株病原真菌的分离、鉴定及碳源代谢指纹图谱分析

Isolation, Identification and Carbon Metabolic Fingerprinting Analysis of Four Pathogens Isolated from Postharvest Plum Fruit

王友升 1陈玉娟 1张燕1

作者信息

  • 1. 北京工商大学食品学院,食品添加剂与配料北京高校工程研究中心,北京市食品风味化学重点实验室,北京100048
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摘要

Abstract

Four pathogens, strain 059#, 067#, 087# and 088#, were isolated from infected plum fruit during postharvest storage. Morphological characterization and rDNA ITS analysis confirmed that the four pathogens were Gibberella moniliformis, Alternaria alternate, Penicillium oxalicum and Aspergillus tubingensis, respectively. A Biolog Microbial Identification System with FF MicroPlate was applied for carbon source utilization tests of the four pathogens based on their abilities to utilize 95 different substrates. The results of carbon metabolic fingerprinting analysis showed that all the isolated pathogens had more than 77 optimal carbon sources, 45 ones in common such as D-fructose, D-glucosamine, sucrose, sorbitol, malic acid and citric acid.

关键词

李果实/病原真菌/ITS/rDNA序列分析/碳源代谢指纹图谱

Key words

plum fruit/fungal pathogen/ITS rDNA sequence analysis/carbon metabolic fingerprinting

分类

轻工纺织

引用本文复制引用

王友升,陈玉娟,张燕..李果实贮藏期间4株病原真菌的分离、鉴定及碳源代谢指纹图谱分析[J].食品科学,2012,33(13):235-239,5.

基金项目

国家“863”计划项目 ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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