食品科学2012，Vol.33Issue(13)：263-266,4.
外源性核苷酸对乙醇致发育毒性的体外干预作用
Intervention Effect of Exogenous Nucleotides on Ethanol-Induced Embryonic Abnormality in Whole Embryo Culture
摘要
Abstract
Objective： to explore the intervention effect of exogenous nucleotides on ethanol-induced embryonic abnormality in whole embryo culture and provide a theoretical reference for future studies. Methods： in vitro post-implantation whole embryo culture was used in this study. Pregnant mice with GD 8.5 were killed by cervical dislocation and the uteri were removed into sterile Hank＇ s solution. The embryos with intact yolk sacs and ectoplacental cones were induced by ethanol, and then subjected to intervention of exogenous nucleotides at the doses of 0.032, 0.80 mg/L and 20.0 mg/L, respectively. The growth and develop- ment of mouse embryos and morphological differentiation of tissues and organs after cultivation for 48 h was evaluated using Maele-Fabry scoring system. Results： an obvious intervention effect was achieved through nucleotide supplementation at the dose of 0.80 mg/L, which could result in an increase of crown-rump length, head length, the differentiation of visceral yolk sac vascular vessels. The scores of flexion, heart, tail neural tube, hindbrain, midbrain, forebrain, otic system, forelimb and branchial arch also revealed a significant increase when compared with ethanol group. Although nucleotide supplementation at the doses of 0.032 mg/L and 20.0 mg/L also exhibited protective effects against ethanol-induced developmental toxicity, the protective effect was weaker when compared with the dose of 0.80 mg/L. Conclusion： nucleotide supplementation at proper dose may be of great benefit for the development of embryos exposed to alcohol in the uterus of mice. Exogenous nucleotides provide an effective strategy for the prevention of ethanol-induced birth defects.关键词
外源性核苷酸/乙醇/全胚胎培养Key words
nucleotides/ethanol/whole embryo culture分类
医药卫生引用本文复制引用
赵洁,赵佳夕,许雅君..外源性核苷酸对乙醇致发育毒性的体外干预作用[J].食品科学,2012,33(13):263-266,4.基金项目
北京市自然科学青年基金项目 （）
