食品科学2012，Vol.33Issue(14)：110-116,7.
顶空固相微萃取-气相色谱-质谱法测定药香型白酒中萜烯类化合物
Quantification of Volatile Terpenoids in Chinese Medicinal Liquor Using Headspace-Solid Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry
摘要
Abstract
An HS-SPME coupled with GC-MS method was developed and applied for the quantification of volatile terpenoids in Chinese medicinal liquor. The effects of SPME fiber type, percentage of ethanol, extraction temperature, and adsorption time on the extraction of volatile terpenoids were evaluated. The optimal SPME extraction parameters were determined as follows： samples were diluted to an alcoholicity of 10% and extracted with 50/30 ~tm DVB/CAR/PDMS fiber for 45 min at 60 ~C. The proposed method exhibited good linearity. An excellent correlation coefficient was observed for 41 analytes, no less than 0.9921. The limits of detection were in the range of 0.0t to 3.62 ~tg/L and the limits of quantification varied from 0.03 to 12.05 lag/L. Both the repeatability and precision RSD values were below 15%. The spike recoveries of 41 volatile terpenoids were in the range of 81.01% to 118.94 %. A total of 41 volatile terpenoids were quantified by this method in base and fished samples of Chinese medicinal liquor＂ Dongjiu＂. Total terpenoid content was higher in Daqu base liquor compared to Xiaoqu base liquor. Seventeen terpenoids including D-camphor were found in Daqu base liquor rather than Xiaoqu base liquor. Regardless of the affecting factors coming from distillation such as acid and heat, the 17 terpenoids were speculated to be mainly derived from Daqu fermented grains, while calamine was found only in Xiaoqu base liquor, suggesting that it is derived from Xiaoqu fermented grains. This study indicates that the HS-SPME coupled with GC-MS method is suitable for the quantitative analysis of terpenoids in Chinese medicinal liquor.关键词
药香型白酒/董酒/萜烯类化合物/顶空固相微萃取/气相色谱-质谱法Key words
Chinese medicinal liquor: Dongjiu liquor: terpenoids/headspace-solid phase microextraction (HS-SPME)/gas chromatography-mass spectrometry (GC-MS)分类
轻工纺织引用本文复制引用
范文来,胡光源,徐岩..顶空固相微萃取-气相色谱-质谱法测定药香型白酒中萜烯类化合物[J].食品科学,2012,33(14):110-116,7.基金项目
国家自然科学基金面上项目 （）
“十一五”国家科技支撑计划项目（2007BAK36802 （）
2008BA163806） （）
黔科合重大专项 （）
