食品科学2012，Vol.33Issue(14)：136-141,6.
超声波提取-高效液相色谱-电喷雾离子化质谱法测定白萝卜中植物生长调节剂
Determination of Plant Growth Regulators in Radish by Ultrasound-Assisted Extraction and Liquid Chromatography-Electrospray Ionization Mass Spectrometry
摘要
Abstract
A new method for the determination of plant growth regulators including 2-naphthoxyacetic acid, 2,4- dicholrophenoxyacetic acid and 1-naphthlcetic acid in radish was developed by liquid chromatography-electrospray ionization mass spectrometry （LC-ESI/MS）. The chromatographic separation was performed on a Diamonsil C18 column （250 mm × 4.6mm, 5μm） by using a mobile phase made up of 0.01 mol/L formic acid-ammonium buffer solution （pH 4） and methanol （30：70, V/V） at a flow rate of 0.7 mL/min. The mass spectromeWic quantitative analysis was based on selective ionic measurement by electro- spray ionization in the negative ion mode. Baseline separation was achieved for three plant growth regulators within 8 min. Good linearity was observed in the range of 0.01 -- 10 ~t g/mL for all of them. The detection limit at a signal to noise ratio of 3 was 0.0018 mg/kg for 2-naphthoxyacetic acid, 0.004 mg/kg for 2,4-dicholrophenoxyacetic acid and 0.003 mg/kg for 1-naphthlcetic acid, respectively. Under optimal conditions, the average recovery rate and relative standard deviation were 77.56%--105.08% and 3.48%--15.88% at spike levels of 0.01, 0.05 mg/kg and 0.50 mg/kg, respectively. Therefore, this method is suitable for the determination of plant growth regulators in radish.关键词
高效液相色谱-电喷雾离子化质谱/超声波提取/植物生长调节剂/白萝卜Key words
liquid chromatography-electrospray ionization mass spectrometry (LC-ESI/MS)/ultrasound-assistedextraction/plant growth regulator/radish分类
化学化工引用本文复制引用
唐莉娟,谭婷,万益群..超声波提取-高效液相色谱-电喷雾离子化质谱法测定白萝卜中植物生长调节剂[J].食品科学,2012,33(14):136-141,6.基金项目
国家自然科学基金地区科学基金项目（20765002 （）
20965005） （）
江西省科技支撑计划项日 （）
南昌大学食品科学与技术国家重点实验室目标导项目 （）
南昌大学食品科学与技术国家重点实验室自由探索项目 （）
